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human β ngf  (R&D Systems)


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    Structured Review

    R&D Systems human β ngf
    Human β Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+%CE%B2+ngf/Recombinant+Human+beta-NGF+Protein/pm41999764-275-45-47
    Average 95 stars, based on 113 article reviews
    human β ngf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Sterility:

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization.
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of prewarmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/mL STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca2+ imaging. ..

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of pre-warmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/ml STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca 2+ imaging. ..

    Recombinant:

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization.
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of prewarmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/mL STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca2+ imaging. ..

    Article Title: A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions
    Article Snippet: .. Commercially-available monomer analytes used for SPR included recombinant mouse and human leptin (R&D Systems), recombinant human β-NGF (R&D Systems) and recombinant human angiopoietin-1 (R&D Systems). ..

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of pre-warmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/ml STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca 2+ imaging. ..

    Article Title: 5-HT 7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome
    Article Snippet: .. Recombinant human β-NGF was purchased from R&D Systems (#256-GF), and human BDNF was purchased from Sigma-Aldrich (#B3795). .. In a separate experiment, RA-treated cells were incubated with 5-HT (1 μM; Sigma) or the 5-HT 7 agonist LP211 (0.1 or 1 μM; Sigma) in culture medium containing reduced serum (2% FBS) for 2 days.

    Article Title: Peripheral nerve-targeting and pain-promoting transcriptomic signatures in early Guillain-Barré syndrome
    Article Snippet: .. DRG bulbs were trimmed, cut into 3mm pieces, and placed in 5mL pre-warmed digestion enzyme containing 1 mg/mL Stemxyme I (Worthington Biochemical, LS004106), 0.1 mg/mL DNAse I (Worthington Biochemical, LS002139), and 10ng/mL recombinant human β-NGF (R&D Systems, 256-GF) in HBSS without calcium and magnesium (Thermo Scientific, 14170-112). ..

    Article Title: Antibodies and derivatives thereof
    Article Snippet: .. Following this pre-incubation period, recombinant human β-NGF (rec-hu-β-NGF, R&D Systems) was added to the cell-antibody mixture. ..

    Article Title: A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics
    Article Snippet: The plate was washed 3x in PBST and 2x PBS and then blocked for 1 hr at room temperature with Blocker TM 1% casein in PBS (Thermo Scientific #37528), followed by 4x washes with PBST and 2x PBS. .. Thirty ng recombinant human β-NGF (R&D Systems, 256-GF/CF) was mixed with 50 μl peptide (Isogenica or Alta Biosciences, UK) at appropriate concentration in 0.1% casein in PBS/0.1% Tween. .. NGF/peptide solutions (55 μl/well) were transferred into the NUNC Maxisorp plate and incubated for 40 minutes at room temperature with 50 μl/well 1:200 diluted biotinylated anti-human β-NGF antibody (R&D Systems, 256-GF/CF) in 0.1% casein in PBS/0.1% Tween.

    Incubation:

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization.
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of prewarmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/mL STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca2+ imaging. ..

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of pre-warmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/ml STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca 2+ imaging. ..

    Immunocytochemistry:

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization.
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of prewarmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/mL STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca2+ imaging. ..

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of pre-warmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/ml STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca 2+ imaging. ..

    Imaging:

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization.
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of prewarmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/mL STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca2+ imaging. ..

    Article Title: Type I interferons enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization
    Article Snippet: .. The hDRGs were minced into small pieces using sterile scissors, immediately transferred to 5 mL of pre-warmed (37°C) STEMxyme 1 (Worthington Biochemical, cat. no. LS004106) solution containing 1 mg/ml STEMxyme in Hank’s Balanced Salt Solution (HBSS), 0.1 mg/mL DNAse I (Worthington Biochemical, cat. no. LS002139), and 10 ng/mL recombinant human β-NGF (R&D Systems, cat. no. 256-GF) and incubated at 37°C in a gently shaking water bath for 8-9 h for ICC and Ca 2+ imaging. ..

    other:

    Article Title: Type I IFNs enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization
    Article Snippet: Data were plotted using GraphPad Prism V9.

    SPR Assay:

    Article Title: A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions
    Article Snippet: .. Commercially-available monomer analytes used for SPR included recombinant mouse and human leptin (R&D Systems), recombinant human β-NGF (R&D Systems) and recombinant human angiopoietin-1 (R&D Systems). ..

    Concentration Assay:

    Article Title: A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics
    Article Snippet: The plate was washed 3x in PBST and 2x PBS and then blocked for 1 hr at room temperature with Blocker TM 1% casein in PBS (Thermo Scientific #37528), followed by 4x washes with PBST and 2x PBS. .. Thirty ng recombinant human β-NGF (R&D Systems, 256-GF/CF) was mixed with 50 μl peptide (Isogenica or Alta Biosciences, UK) at appropriate concentration in 0.1% casein in PBS/0.1% Tween. .. NGF/peptide solutions (55 μl/well) were transferred into the NUNC Maxisorp plate and incubated for 40 minutes at room temperature with 50 μl/well 1:200 diluted biotinylated anti-human β-NGF antibody (R&D Systems, 256-GF/CF) in 0.1% casein in PBS/0.1% Tween.



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    Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced <t>by</t> <t>stimulation</t> with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , <t>NGF</t> , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.
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    Image Search Results


    Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis

    doi: 10.1038/s41467-026-70000-6

    Figure Lengend Snippet: Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

    Article Snippet: The mixture was placed in an incubator at 37 °C with 5% CO 2 for 30 min, followed by stimulation with recombinant human NGF (R&D Systems, 256-GF) at a final concentration 0.1 μg/mL (or PBS as negative control) and the resulting mixtures were further incubated for 20 min. Next, anti-CD123-APC (Miltenyi Biotec, 130-115-265) and anti-CD203c-PE (Miltenyi Biotec, 130-092-343) antibodies were added and continued to incubate for another 15 minutes.

    Techniques: RNA Expression, Control, Expressing, RNA Sequencing